![]() Several reviews have been published about gene editing in pigs, from biomedical and agricultural standpoint ( Burkard et al., 2018 Yang and Wu, 2018 Lee et al., 2019). In the last few years, there has been a huge impact on porcine biotechnology evolution, evidenced by the numerous pig models developed in this short period of time. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. ![]() ![]() Pigs are an important resource for meat production and serve as a model for human diseases. ![]()
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